Star-Lord - Cannonball!

Cannonball has some serious potential and this is an attempt to use it.

10 of the other cards in this deck have the Aerial trait. While nowhere near as good as Angel, roughly 1 in 4 cards you draw will have the trait to help him reduce consequential damage, just keeping one in hand at the end of your turn can make a consequential damage-free turn for Cannonball on your next turn much more likely. Star-Lord's Helmet can help with this, at the cost of more encounter cards, but you might want that anyway to benefit from some of his other cards.

All of the ally upgrades here are intended for Cannonball. Inspired, Laser Blaster, and Sidearm increase his stats, while Reinforced Suit and Team Training let him take the occasional point of damage. Leader of the Guardians and Cloud 9 also boost his stats. Getting everything out would result in a THW 5/ATK 5 ally with ranged and overkill who has the ability to not take consequential damage.

C.I.T.T. allows him to activate twice in a turn. If he does get used up, Med Lab can bring him back (although he would have to start over on his upgrades). Call for Backup can fish him out of your deck or discard pile.

While this seems very "all your eggs in one basket", the combo of Star-Lord's ability, "Welcome Aboard" and The Power of Flight can get out allies super cheap, allowing you to make use of Knowhere and your increased ally limit and card draw (remember that all allies are Guardians with Star-Lord), then slap enemies around with Air Supremacy.

If you want to trim down to 40 cards, I would recommend cutting Reinforced Suit, 1 copy of The Power of Flight and 1 copy of "Welcome Aboard"

Remember that your allies are here to get used up and cycled back into the deck to fuel more Cannonball antics or Med Lab to be brought back into action quickly. Captain Marvel, Falcon, Nova Prime, Sunspot, and Vivian all provide a benefit on entering play, so recycling them through the Med Lab are your most beneficial options.

Victory:

(Solo) Standard Zola w/Black Tom Cassidy